EB Group Patents

Novel In-Vessel High Rate Composter

Inventor

Mohd Ali Hassan

IP Status

Pending Patent

Filed Date

17 December 2010

Application Number

PI/2010/006055

Country Filing

MALAYSIA

Applicant

Universiti Putra Malaysia, Ecobuilders Sdn. Bhd.

Abstract

The present invention is an-in-vessel high rate composting apparatus for processing of oil palm biomass, organic and municipal wastes to produce compost product. The composting apparatus consists of a vertical position and cylindrical shape vessel (100) with conical bottom (2) design, materials feeding (1) and product discharge systems (3), a screw impeller (12) for axial mixing, aeration (14) and carbon dioxide (CO2) removal systems and a leachate collection system (15). The optimum composting conditions in the vessel will be maintained by means of programming logic controller (PLC) on key parameters such as temperature, oxygen (O2) level, moisture level and carbon dioxide (CO2) level. The compost product could be let to mature inside the vessel or let to cure elsewhere for the completion of the compost product.

A Method for Recovering an Intracellular Polyhydroxyalkanoates (PHA)

Inventor

Mohd Ali Hassan, Mitra Mohammadi, Nor Asma Abd Razak, Chong Mei Ling, Yoshihito Shirai, Wan Md Zin Wan Yunus, Suraini Abd. Aziz, Amirul Al-Ashraf Abdullah, Hasfalina Che Man, Siti Nor Syairah Anis, Phang Lai Yee

IP Status

Pending Patent

Filed Date

01 March 2010

Application Number

PI/2010/000891

Country Filing

Malaysia

Applicant

Universiti Putra Malaysia

Abstract

The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from setp (a) with distilled water, (c) freeze-drying pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM)from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing fi nal pellet, and (g) freeze-drying the pellet produced from step (f).

A Novel Bacterium Producing Polyhydroxyalkanoates from Palm Oil Mill Effluent

Inventor

Mohd. Ali Hassan, Mohd. Rafein Zakaria, Suriani Abd. Aziz, Farinazleen Mohamad Ghazali, Yoshihito Shirai, Minato Wakisaka

IP Status

Pending Patent

Filed Date

07 January 2010

Application Number

PCT/MY2010/000004

Country Filing

Malaysia

Applicant

Universiti Putra Malaysia

Abstract

The bacterial strain EB 172, isolated from digester treating palm oil mill effl uent, was investigated by polyphasic taxonomic approach. The cells were 5 rod-shaped, Gramnegative,non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strain was placed in the cluster of genus Comamonas; its closest neighbours were the type strains C. terrigena (96.8 %), C. koreensis (93.4 %), C. composti(92.9 %), and C. kerstersii(91.1 %). The 10 ability of C. putranensis to produce polyhydroxyalkanoates (PHA) when supplied with organic acids made this bacterium is unique in Comamonas species. The bacterial strain was clearly distinguished from all of the existing strains using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics. The DNA G+C content of the genomic DNA was 59.1 mol%. It is evident 15 from the genotypic and phenotypic data that strain Comamonas putranensis represents a novel species in the genus Comamonas, for which the name Comamonas putranensis sp.nov. is proposed.

Method for Isolating DNA

Inventor

Mohd Ali Hassan, Meisam Tabatabaei, Mohd Rafein Zakaria, Raha Abdul Rahim, Andre-Denis G.Wright, Yoshihito Shirai, Norhani Abdullah, Mehdi Shamsara, Kenji Sakai

IP Status

Pending Patent

Filed Date

11 September 2009

Application Number

PCT/MY2009/000143

Country Filing

PCT

Applicant

Universiti Putra Malaysia

Abstract

The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsufate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells.

A Novel Bacterium Producing Polyhydroxyalkanoates
from Palm Oil Mill Effluent

Inventor

Mohd. Ali Hassan, Mohd. Rafein Zakaria, Suriani Abd. Aziz, Farinazleen Mohamad Ghazali, Yoshihito Shirai, Minato Wakisaka

IP Status

Pending Patent

Filed Date

29 June 2009

Application Number

PI20092761

Country Filing

MALAYSIA

Applicant

Universiti Putra Malaysia, Felda Palm Industries Sdn. Bhd.,
Kyushu Institute of Technology

Abstract

The bacterial strain EB 172, isolated from digester treating palm oil mill effluent, was investigated by polyphasic taxanomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analyses using the 16S rRNA gene sequence showed that the strains C. terrigena (96.8%), C. koreensis (93.4%), C. composti (92.9%), and C.kerstersii (91.1%). The ability of C. putranesis to produce polyhydroxyalkanoates (PHA) when supplied with organic acids made this bacterium is unique in Comamonas species. The bacterial strain was clearly distinguished from all of the existing strains using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics. The DNA G+C content of the genomic DNA was 59.1 mol%. It is evident from the genotypic and phenotypic data that strain Comamonas putranesis represents a novel species in the genus Comamonas, for which the name Comamonas putranesis sp. nov. is proposed.

A Method of Extracting and
Purifying Polyhydroxyalkanoate Bioplastic

Inventor

Mohd Ali Hassan, Voon Phooi Tee, Phang Lai Yee, Yoshihito Shirai

IP Status

Pending Patent

Filed Date

18 December 2008

Application Number

PI20085129

Country Filing

MALAYSIA

Applicant

Universiti Putra Malaysia

Abstract

Polyhydroxyalkanoates (PHA) is an intracellular polymer that is biologically synthesized by microorganisms as carbon and energy reverse material. This biodegradable material has properties similar to petrochemical thermoplastics. Currently methods of PHA extraction and purification from bacterial suspension use solvent or halogenated-based approaches which are costly and pose severe environmental damage. This invention describes a combined treatment of alkaline and homogenization for extracting and purifying PHA, with higher PHA purity and recovery.

A Process for Treatment of Palm Oil Mill Effluent
and for Conversion of the Palm Oil Mill Effluent Into
Biodegradable Plastics

Inventor

Mohd Ali Hassan

IP Status

Granted Patent

Filed Date

6 April 1996

Granted Date

31 May 2005

Application Number

PI96001322

Country Filing

MALAYSIA

Applicant

Universiti Putra Malaysia

IP Number

MY123658A

Technology

Waste Management, Biodegradable

Abstract

A process is described for converting organic materials from liquid oil mill affluent into a bioplastic suitable for use as a biodegradable plastic. In a preferred embodiment the process involves anaerobic treatment of palm oil moll effluent (POME) with digested sludge at pH of about to produce organic acid, particularly acetic and propionic, the solid of POME is removed by sodium hydroxide precipitation at pH of about 12. The clarified fraction of POME is then inoculated with photosynthesis bacterium of Rhodobacter species which accumulates the bioplastic by the cells utilising the organic acids produced and certrifugation by drying and solvent extraction for the recovery of the bioplastic.